• Flowjo Compensation Calculation Error

    FlowJo vX Lessons - 2 - CompensationPDF Three Rules for Compensation Controls (From The Daily Dongle … – … http://flowjo.typepad.com/the_daily_dongle/2011/09/three-rules-for- … The error in the compensation coefficient is the sum of the absolute … compensation spillover calculation will be zero. If this is met, …

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    SOP015 Flowjo CFC-CRP-Santé Page 1 sur 5 Confirmed Original (Signature author in red) Flow Data Analysis with Flowjo (based on version 7.6) SOP015 02/03 … The Diva compensation is imported into Flowjo with the FCS files. In the Flowjo

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    If you save a compensation matrix from flowjo and open the file In a text editor … so small absolute errors in the position of the negative control become irrelevant as the positive controls become … It is imperative to NOT include autofluorescence in the compensation calculation, …

    Chapter 1 – Editing Acquisition-Defined Compensation Matrices. CHAPTER 1 … They are read and applied by FlowJo to the files automatically when the files are loaded. … Visual manipulations will likely only introduce error in the compensation calculation.

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    Using FlowJo to calculate compensation 060502 Emory Vaccine Center FCC / Suzanne Mertens / saguila@emory.edu FlowJo can be used to calculate compensation for multi-color data acquired on a Calibur Organize your files into groups First place your “comp” tubes into a separate group The put …

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